Lorenzo Galluzzi, Guido Kroemer's Cell-wide metabolic alterations associated with malignancy PDF

By Lorenzo Galluzzi, Guido Kroemer

ISBN-10: 012801329X

ISBN-13: 9780128013298

This new quantity of tools in Enzymology keeps the legacy of this most advantageous serial with caliber chapters authored by way of leaders within the box. This quantity covers examine tools delivering a a theoretical review on metabolic adjustments of melanoma cells and a sequence of protocols that may be hired to check oncometabolism, in vitro, ex vivo and in vivo.   Malignant cells show metabolic adjustments compared to their general opposite numbers, because of either genetic and epigenetic adjustments. even though the sort of metabolic rewiring has lately been indicated as ''yet another'' normal hallmark of melanoma, gathering facts means that the metabolic changes of every neoplasm really signify a molecular signature that in detail accompanies, and therefore can't be severed from, all elements of malignant transformation.

  • Continues the legacy of this most excellent serial with caliber chapters authored by way of leaders within the field
  • Covers study equipment in biomineralization science
  • Contains sections on such themes supplying a a theoretical evaluation on metabolic adjustments of melanoma cells and a sequence of protocols that may be hired to check oncometabolism, in vitro, ex vivo and in vivo.

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2A). During the measurements, the photomultiplier is placed in close proximity to the coverslip with the transfected cells (Fig. 2B and C). 2 Measurements and calibration After aequorin reconstitution and immediately before performing the experiment, the coverslip with the transfected cells is transferred to the perfusion chamber of the luminometer. Cells are maintained at 37  C and continuously perfused with KRB medium by a peristaltic pump connected with the thermostatted chamber. , 1995). 3 shows typical Ca2+ measurements obtained in HeLa cells transfected with cytAEQ, erAEQ, and mtAEQmut, respectively.

A critical evaluation. Journal of Biological Chemistry, 270(17), 9896–9903. , & Rizzuto, R. (1994). Nuclear targeting of aequorin. A new approach for measuring nuclear Ca2+ concentration in intact cells. Cell Calcium, 16(4), 259–268. , & Rizzuto, R. (1993). Nuclear Ca2+ concentration measured with specifically targeted recombinant aequorin. EMBO Journal, 12(12), 4813–4819. Chalfie, M. (1995). Green fluorescent protein. Photochemistry and Photobiology, 62(4), 651–656. Cobbold, P. , & Lee, J. A. C.

2013), opened the possibility to directly manipulate mitochondrial Ca2+ fluxes and to study the consequences. Very recently, the role of MCU in cancer cells survival and in cell resistance to apoptotic stimuli has been investigated by Pinton and coworkers. They have identified a family of cancer-related miRNA (miRNA-25) able to regulate the expression level of MCU by promoting its degradation, thus conferring apoptotic resistance. , 2013). 2. Aequorin: From the jellyfish to the transgenic mice Aequorin is a 21 kDa photoprotein produced by the jellyfish Aequorea victoria composed by an apoprotein and a prosthetic group, the coelenterazine.

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Cell-wide metabolic alterations associated with malignancy by Lorenzo Galluzzi, Guido Kroemer


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